Keep as concentrated solution.
Positive Control: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, human ESC, NIH-3T3, BCL2, Rat2, OC3.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: acute phase response factor; Acute-phase response factor; acute-phase response factor antibody; APRF antibody; DNA-binding protein APRF; DNA-binding protein APRF antibody; FLJ20882 antibody; HIES antibody; MGC16063 antibody; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3 (acute-phase response factor) antibody; signal transducer and activator of transcription 3 antibody; STAT3 antibody
Gene Aliases: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3