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STAT5 beta Antibody (710139) in WB
Western blot analysis of STAT5B was performed by loading 20 µg of HeLa (lane1) and K562 (lane2) cell lysates using Novex®NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Proteins were transferred to a PVDF membrane and blocked with 5 % skim milk for 1 hour at room temperature. STAT5 Beta was detected at ~90 kDa using STAT5B Recombinant Rabbit Monoclonal Antibody (Product # 710139) at 1 µg-3 µg/mL in 2.5 % skim milk at 4... View More
Product Details
710139
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
13HCLC
Immunogen
Peptide corresponding to amino acids 49-57 of human STAT5b
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Signal transducer and activator of transcription 5B; STA5B; STAT; transcription factor STAT5B
Gene Aliases: GHISID2; STAT5; STAT5B
UniProt ID: (Human) P51692
Entrez Gene ID: (Human) 6777
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
glucocorticoid receptor binding
identical protein binding
protein homodimerization activity
protein dimerization activity
mitotic cell cycle
luteinization
natural killer cell differentiation
natural killer cell proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
defense response
signal transduction
JAK-STAT cascade
sex differentiation
female pregnancy
lactation
cell proliferation
positive regulation of cell proliferation
regulation of steroid metabolic process
cytokine-mediated signaling pathway
taurine metabolic process
lipid storage
lymphocyte differentiation
regulation of cell adhesion
B cell differentiation
erythrocyte differentiation
regulation of epithelial cell differentiation
response to estradiol
positive regulation of interleukin-2 production
positive regulation of natural killer cell proliferation
positive regulation of natural killer cell differentiation
cellular response to hormone stimulus
myeloid cell apoptotic process
negative regulation of myeloid cell apoptotic process
T cell differentiation in thymus
erythropoietin-mediated signaling pathway
regulation of multicellular organism growth
positive regulation of multicellular organism growth
positive regulation of activated T cell proliferation
regulation of cell proliferation
natural killer cell mediated cytotoxicity
progesterone metabolic process
gamma-delta T cell differentiation
T cell homeostasis
response to peptide hormone
positive regulation of B cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of lymphocyte differentiation
negative regulation of erythrocyte differentiation
positive regulation of erythrocyte differentiation
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
positive regulation of natural killer cell mediated cytotoxicity
development of secondary female sexual characteristics
development of secondary male sexual characteristics
Peyer's patch development
positive regulation of inflammatory response
activated T cell proliferation
JAK-STAT cascade involved in growth hormone signaling pathway
response to interleukin-2
response to interleukin-4
response to interleukin-15
cellular response to growth factor stimulus
cellular response to epidermal growth factor stimulus
mast cell migration
regulation of hemopoiesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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