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STIM1 Antibody (600-601-J49) in IHC (P)
Immunohistochemistry of Sheep anti-GOK / STIM1 antibody. Tissue: Thyroid. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Product Details
600-601-J49
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Stim 1 affinity purified antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human Stim 1.
Conjugate
Form
Liquid
Concentration
1.12 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 1mg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.^M
^M
A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: STIM1L; Stromal interaction molecule 1
Gene Aliases: D11S4896E; GOK; IMD10; STIM1; STRMK; TAM; TAM1
UniProt ID: (Human) Q13586
Entrez Gene ID: (Human) 6786
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