|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to a C-terminal region with residues 600-685 of the human protein.[Uniprot#Q13586]|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 93% sequence homology with porcine and 79% sequence homology with xenopus.
Suggested positive control: antigen standard for STIM1 (transient overexpression lysate), HeLa whole cell lysate.
Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic recticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.