|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region of the human Stk33 (within residues 1-150).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Serine Threonine Kinase 33 is normally expressed in embryonic and germ-line cells, which ultimately differentiate into testis, lung, heart, brain and spinal cord. It is critical in both spermatogenesis and organ ontogenesis, and is thus a critical developmental protein. However, in many malignant tissues, STK33 is abnormally expressed, and its appearance in these tissues usually indicates an increase in the cancer cell viability. In cancer cells, STK33 suppresses mitochondrial apoptosis, which precludes normal processes to identify and eliminate cancerous cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.