Invitrogen

STMN2 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (19) STMN2 antibodies

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Protocols

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STMN2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence was performed on fixed and permeabilized SHSY-5Y cells for detection of Stathmin-2 using Anti-Stathmin-2 Rabbit Polyclonal Antibody (Product # 720178, 1 µg/mL), alpha-Tubulin was detected using Anti-alpha Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor®594conjugate (Product # A-11032, 1:400) respectively. Panel a) shows representative cells that were stained for detection and localization of Stathmin-2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal alpha-tubulin staining (red). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Stathmin-2. Panel e) represents control cells with no primary Antibody to assess background.

STMN2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), Rat Brain (Lane 3) and Mouse Brain (Lane4). The blots were probed with Anti- SCG10/Stathmin-2 Rabbit Polyclonal Antibody (Product # 720178, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 21 kDa band corresponding to SCG10/Stathmin-2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

STMN2 Antibody — Figure 4 Silencing Stathmin-2 increased glucagon secretion and overexpression of stathmin-2 suppressed glucagon secretion in alphaTC1-6 cells. Wild type (wt; control) and stathmin-2 depleted (Stmn2-KD) aTC1-6 cells were pre-incubated 2 h in serum-free medium and then incubated with or without KCl (55 mM) for 15 min. (A) Glucagon secretion is significantly stimulated by KCL in wt cells, while in Stmn-KD cells, basal glucagon secretion is increased and does not respond to KCl. * p < 0.01 compared to basal secretion in wt cells. (B) Stmn2 mRNA levels are decreased by about 70% after siRNA-mediated depletion in alphaTC1-6 cells. Values are means +- SEM ( n = 5), * p < 0.01. (C) Stathmin-2 protein levels are depleted after siRNA-mediated silencing of Stmn2. C (control); KD (gene silenced). Beta-actin was used as a loading control. (D) Proglucagon mRNA levels are not affected by siRNA-mediated depletion of stathmin-2. (E) Glucagon secretion is inhibited by overexpression of Stmn2. alphaTC1-6 cells were transfected with pcDNA3.1 (+) MAr-stmn2 or empty vector (negative control). Both basal and K + -stimulated glucagon secretion were inhibited by overexpression of Stmn2. Values are means +- SEM ( n = 4). * p < 0.05; ** p < 0.001 compared to unstimulated control. {KD}

Product Details

720178

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-1 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human STMN2 (aa 29-179) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2606219

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Target Information

STMN2, also known as SCG-10, is a neuronal growth-associated protein that shares significant amino acid sequence similarity with the phosphoprotein stathmin. STMN2 may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stathmin-2 Mediates Glucagon Secretion From Pancreatic α-Cells.

Frontiers in endocrinology

Asadi F,Dhanvantari S

720178 was used in Western Blotting to describe a potential new pathway for the regulation of glucagon secretion, and may have implications for controlling glucagon hypersecretion in diabetes.

Wed Feb 03 00:00:00 EST 2021

The landscape of multiscale transcriptomic networks and key regulators in Parkinson's disease.

Nature communications

Wang Q,Zhang Y,Wang M,Song WM,Shen Q,McKenzie A,Choi I,Zhou X,Pan PY,Yue Z,Zhang B

720178 was used in Western Blotting to develop multiscale gene network models of Parkinson's disease and identify potential key regulators of the disease which require further investigation.

Wed Nov 20 00:00:00 EST 2019

The landscape of multiscale transcriptomic networks and key regulators in Parkinson's disease.

Nature communications

Wang Q,Zhang Y,Wang M,Song WM,Shen Q,McKenzie A,Choi I,Zhou X,Pan PY,Yue Z,Zhang B

720178 was used in Western Blotting to develop multiscale gene network models of Parkinson's disease and identify potential key regulators of the disease which require further investigation.

Wed Nov 20 00:00:00 EST 2019

Bioinformatics

Protein Aliases: neuron-specific growth-associated protein; neuronal growth-associated protein (silencer element); Protein SCG10; SGC10; Stathmin-2; stathmin-like 2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein; superiorcervical ganglia, neural specific 10

Gene Aliases: AI159727; SCG10; SCGN10; Stmb2; STMN2

UniProt ID: (Human) Q93045, (Rat) P21818, (Mouse) P55821

Entrez Gene ID: (Human) 11075, (Rat) 84510, (Mouse) 20257

Function(s)

negative regulation of microtubule depolymerization positive regulation of neuron projection development negative regulation of neuron projection development negative regulation of microtubule polymerization positive regulation of microtubule depolymerization cellular response to nerve growth factor stimulus microtubule depolymerization neuron projection development regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization

Process(es)

protein binding tubulin binding calcium-dependent protein binding

STMN2 Polyclonal Antibody

Product Details

720178

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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