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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • STS Antibodies

          Invitrogen

          STS Polyclonal Antibody

          View all (11) STS antibodies

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          Cite STS Polyclonal Antibody

          • Antibody Testing Data (2)
          STS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          STS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          STS Antibody (PA5-80083) in WB

          Western blot analysis of STS in Lane 1: human HeLa whole cell lysate, Lane 2: human placenta tissue lysate, Lane 3: human MCF-7 whole cell lysate, Lane 4: human HepG2 whole cell lysate, Lane 5: human PANC-1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          STS Antibody in Western Blot (WB)
          STS Antibody in Flow Cytometry (Flow)
          STS Polyclonal Antibody

          Product Details

          PA5-80083

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2747198

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human HepG2 whole cell, human PANC-1 whole cellFlow: Hela cells.

          Target Information

          The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: Arylsulfatase C; ASC; Estrone sulfatase; Steroid sulfatase; Steryl-sulfatase; Steryl-sulfate sulfohydrolase

          View more View less

          Gene Aliases: ARSC; ARSC1; ASC; ES; SSDD; STS; XLI

          View more View less

          UniProt ID: (Human) P08842, (Mouse) P50427, (Rat) P15589

          View more View less

          Entrez Gene ID: (Human) 412, (Mouse) 20905, (Rat) 24800

          View more View less

          Function(s)
          steryl-sulfatase activity sulfuric ester hydrolase activity metal ion binding catalytic activity hydrolase activity hydrolase
          Process(es)
          glycosphingolipid metabolic process steroid catabolic process female pregnancy learning or memory positive regulation of cell proliferation epidermis development response to pH response to organic cyclic compound response to peptide hormone skin development response to estrogen post-translational protein modification lipid metabolic process metabolic process steroid metabolic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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