Invitrogen

STX17 Polyclonal Antibody

1 Published Figure

2 References

View all (6) STX17 antibodies

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Datasheet

Questions & Answers

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STX17 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Syntaxin-17 in HeLa cells. Samples were fixed in 4% paraformaldehyde at RT for 15 min and probed with a Syntaxin-17 polyclonal antibody (Product # PA5-40127) at a dilution of 1:200 (green). Nuclei were stained using Hoechst 33342 (blue). Scale bar = 10 µm.

STX17 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of STX17 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: STX17 Polyclonal Antibody (Product # PA5-40127) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

STX17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Syntaxin-17 in paraffin-embedded mouse prostate tissue using a Syntaxin-17 polyclonal antibody (Product # PA5-40127) at a dilution of 1:500.

STX17 Antibody in Western Blot (WB) — Western Blot analysis of STX17 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a STX17 Polyclonal Antibody (Product # PA5-40127) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

STX17 Antibody — Fig. 1 Mitochondrial fission 1 protein (Fis1) and syntaxin 17 (STX17) interact and partially colocalize. a , b HeLa cells were transfected with Flag-tagged vector or Fis1. After 24 h, cells were collected for immunoprecipitation (IP) with anti-Flag beads. Coomassie blue staining was used to visualize bands 1 and 2 ( a ). Results for mass spectrometry analysis of band 1 and 2 are summarized ( b ). c Cells treated as in a were extracted. Anti-Flag immunoprecipitates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted for STX17 and Flag. Asterisk indicates a non-specific band. d HEK293T cells were co-transfected with Myc-tagged STX17 and Flag-tagged plasmids as indicated. Cells were solubilized for IP with anti-Flag and analyzed with Myc and Flag antibodies respectively. e HeLa cells were transfected with green fluorescent protein (GFP)-tagged vector or STX17 (green) and mCherry-tagged vector or plasmid encoding Fis1 (red) for 24 h. Cells were fixed and stained with anti-Tom20 (cyan). Hoechst, blue. Scale bar, 10 um. f HeLa cells were treated with the indicated small interfering RNA (siRNA) for 24 h before transfecting with GFP-tagged Fis1 (green) or GFP-STX17 (green) for further 24 h. Representative confocal images of live cells are shown. Mitochondrial morphology was visualized using MitoTracker Red (MTR, red). Scale bar, 10 um. White arrowhead indicates cells with decreased MTR. g Quantification of cells with

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1,000

View 1 publication 1 publication

Immunofluorescence (IF)

1:100-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

View 1 publication 1 publication

Product Specifications

Species

Human, Mouse, Rat

Published species

Artificial Control, Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human STX17.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2608436

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.

Predicted reactivity: Mouse (89%), Rat (86%), Rhesus Monkey (99%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.