Invitrogen

STX17 Polyclonal Antibody

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STX17 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of STX17 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: STX17 Polyclonal Antibody (Product # PA5-40127) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

STX17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Syntaxin-17 in paraffin-embedded mouse prostate tissue using a Syntaxin-17 polyclonal antibody (Product # PA5-40127) at a dilution of 1:500.

STX17 Antibody in Western Blot (WB) — Western Blot analysis of STX17 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a STX17 Polyclonal Antibody (Product # PA5-40127) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-40127

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human STX17.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.85 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2608436

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.

Predicted reactivity: Mouse (89%), Rat (86%), Rhesus Monkey (99%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi. (Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Nature communications

Xian H,Yang Q,Xiao L,Shen HM,Liou YC

PA5-40127 was used in Immunocytochemistry-immunoflourescence to demonstrate that the SNARE protein Syntaxin 17 initiates mitophagy upon depletion of outer mitochondrial membrane protein Fis1.

Fri May 03 00:00:00 EDT 2019

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Nature communications

Xian H,Yang Q,Xiao L,Shen HM,Liou YC

PA5-40127 was used in Immunocytochemistry-immunoflourescence to demonstrate that the SNARE protein Syntaxin 17 initiates mitophagy upon depletion of outer mitochondrial membrane protein Fis1.