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SUCLA2 Polyclonal Antibody

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Antibody Testing Data (20)

SUCLA2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

SUCLA2 Antibody (12627-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12627-1-AP (SUCLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SUCLA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SUCLA2 Antibody in Immunoprecipitation (IP)

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Product Details

12627-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SUCLA2 Fusion Protein Ag3319 (24-353 aa encoded by BC027587)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AQRAAAQVL GSSGLFNNHG LQVQQQQQRN LSLHEYMSME LLQEAGVSVP KGYVAKSPDE AYAIAKKLGS KDVVIKAQVL AGGRGKGTFE SGLKGGVKIV FSPEEAKAVS SQMIGKKLFT KQTGEKGRIC NQVLVCERKY PRREYYFAIT MERSFQGPVL IGSSHGGVNI EDVAAESPEA IIKEPIDIEE GIKKEQALQL AQKMGFPPNI VESAAENMVK LYSLFLKYDA TMIEINPMVE DSDGAVLCMD AKINFDSNSA YRQKKIFDLQ DWTQEDERDK DAAKANLNYI GLDGNIGCLV NGAGLAMATM DIIKLHGGTP ANFLDVGGGA T (24-353 aa encoded by BC027587)

Target Information

ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 EDT 2023

Investigations into photoreceptor energy metabolism during experimental retinal detachment.

Frontiers in cellular neuroscience

Chidlow G,Chan WO,Wood JPM,Casson RJ

Tue Dec 06 00:00:00 EST 2022

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.

Aging and disease

Yuan M,Wang Y,Wen J,Jing F,Zou Q,Pu Y,Pan T,Cai Z

Sat Oct 01 00:00:00 EDT 2022

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation.

Molecular cell

Trefely S,Huber K,Liu J,Noji M,Stransky S,Singh J,Doan MT,Lovell CD,von Krusenstiern E,Jiang H,Bostwick A,Pepper HL,Izzo L,Zhao S,Xu JP,Bedi KC,Rame JE,Bogner-Strauss JG,Mesaros C,Sidoli S,Wellen KE,Snyder NW

Thu Jan 20 00:00:00 EST 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

Oncogene

Pang Z,Dong X,Deng H,Wang C,Liao X,Liao C,Liao Y,Tian W,Cheng J,Chen G,Yi H,Huang L

Sun May 01 00:00:00 EDT 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

Oncogene

Pang Z,Dong X,Deng H,Wang C,Liao X,Liao C,Liao Y,Tian W,Cheng J,Chen G,Yi H,Huang L

Sun May 01 00:00:00 EDT 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

Oncogene

Pang Z,Dong X,Deng H,Wang C,Liao X,Liao C,Liao Y,Tian W,Cheng J,Chen G,Yi H,Huang L

Sun May 01 00:00:00 EDT 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

Oncogene

Pang Z,Dong X,Deng H,Wang C,Liao X,Liao C,Liao Y,Tian W,Cheng J,Chen G,Yi H,Huang L

Sun May 01 00:00:00 EDT 2022

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Nature communications

Wang XH,Xu S,Zhou XY,Zhao R,Lin Y,Cao J,Zang WD,Tao H,Xu W,Li MQ,Zhao SM,Jin LP,Zhao JY

Tue Jun 08 00:00:00 EDT 2021

Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.

Oncogene

Kohno S,Linn P,Nagatani N,Watanabe Y,Kumar S,Soga T,Takahashi C

Sat Aug 01 00:00:00 EDT 2020

Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.

Oncogene

Kohno S,Linn P,Nagatani N,Watanabe Y,Kumar S,Soga T,Takahashi C

Sat Aug 01 00:00:00 EDT 2020

Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.

Brain structure & function

Dobolyi A,Bago A,Palkovits M,Nemeria NS,Jordan F,Doczi J,Ambrus A,Adam-Vizi V,Chinopoulos C

Sun Mar 01 00:00:00 EST 2020

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Haematologica

Maio N,Kim KS,Holmes-Hampton G,Singh A,Rouault TA

Sun Sep 01 00:00:00 EDT 2019

Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients.

Journal of inherited metabolic disease

Carrozzo R,Verrigni D,Rasmussen M,de Coo R,Amartino H,Bianchi M,Buhas D,Mesli S,Naess K,Born AP,Woldseth B,Prontera P,Batbayli M,Ravn K,Joensen F,Cordelli DM,Santorelli FM,Tulinius M,Darin N,Duno M,Jouvencel P,Burlina A,Stangoni G,Bertini E,Redonnet-Vernhet I,Wibrand F,Dionisi-Vici C,Uusimaa J,Vieira P,Osorio AN,McFarland R,Taylor RW,Holme E,Ostergaard E

Tue Mar 01 00:00:00 EST 2016

Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients.

Journal of inherited metabolic disease

Tue Mar 01 00:00:00 EST 2016

Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations.

The Biochemical journal

Kacso G,Ravasz D,Doczi J,Németh B,Madgar O,Saada A,Ilin P,Miller C,Ostergaard E,Iordanov I,Adams D,Vargedo Z,Araki M,Araki K,Nakahara M,Ito H,Gál A,Molnár MJ,Nagy Z,Patocs A,Adam-Vizi V,Chinopoulos C

Sat Oct 15 00:00:00 EDT 2016

Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations.

The Biochemical journal

Sat Oct 15 00:00:00 EDT 2016

Knockdown of Sucla2 decreases the viability of mouse spermatocytes by inducing apoptosis through injury of the mitochondrial function of cells.

Folia histochemica et cytobiologica

Huang S,Wang J,Wang L

Mon Apr 03 00:00:00 EDT 2017

Knockdown of Sucla2 decreases the viability of mouse spermatocytes by inducing apoptosis through injury of the mitochondrial function of cells.

Folia histochemica et cytobiologica

Huang S,Wang J,Wang L

Mon Apr 03 00:00:00 EDT 2017

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 EDT 2015

Synaptic mitochondria: a brain mitochondria cluster with a specific proteome.

Journal of proteomics

Völgyi K,Gulyássy P,Háden K,Kis V,Badics K,Kékesi KA,Simor A,Györffy B,Tóth EA,Lubec G,Juhász G,Dobolyi A

Wed Apr 29 00:00:00 EDT 2015

Localization of SUCLA2 and SUCLG2 subunits of succinyl CoA ligase within the cerebral cortex suggests the absence of matrix substrate-level phosphorylation in glial cells of the human brain.

Journal of bioenergetics and biomembranes

Dobolyi A,Bagó AG,Gál A,Molnár MJ,Palkovits M,Adam-Vizi V,Chinopoulos C

Wed Apr 01 00:00:00 EDT 2015

Exclusive neuronal expression of SUCLA2 in the human brain.

Brain structure & function

Dobolyi A,Ostergaard E,Bagó AG,Dóczi T,Palkovits M,Gál A,Molnár MJ,Adam-Vizi V,Chinopoulos C

Thu Jan 01 00:00:00 EST 2015

Exclusive neuronal expression of SUCLA2 in the human brain.

Brain structure & function

Dobolyi A,Ostergaard E,Bagó AG,Dóczi T,Palkovits M,Gál A,Molnár MJ,Adam-Vizi V,Chinopoulos C

Thu Jan 01 00:00:00 EST 2015

2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.

Reproductive toxicology (Elmsford, N.Y.)

Huang S,Cui Y,Guo X,Wang L,Li S,Lu Y,Bi Y,Huang X,Lin M,Xia Y,Wang S,Wang X,Zhou Z,Sha J

Thu Jan 01 00:00:00 EST 2015

2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.

Reproductive toxicology (Elmsford, N.Y.)

Huang S,Cui Y,Guo X,Wang L,Li S,Lu Y,Bi Y,Huang X,Lin M,Xia Y,Wang S,Wang X,Zhou Z,Sha J

Thu Jan 01 00:00:00 EST 2015

The negative impact of α-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kiss G,Konrad C,Doczi J,Starkov AA,Kawamata H,Manfredi G,Zhang SF,Gibson GE,Beal MF,Adam-Vizi V,Chinopoulos C

Sat Jun 01 00:00:00 EDT 2013

The negative impact of α-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kiss G,Konrad C,Doczi J,Starkov AA,Kawamata H,Manfredi G,Zhang SF,Gibson GE,Beal MF,Adam-Vizi V,Chinopoulos C

Sat Jun 01 00:00:00 EDT 2013

Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chinopoulos C,Gerencser AA,Mandi M,Mathe K,Töröcsik B,Doczi J,Turiak L,Kiss G,Konràd C,Vajda S,Vereczki V,Oh RJ,Adam-Vizi V

Thu Jul 01 00:00:00 EDT 2010

Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chinopoulos C,Gerencser AA,Mandi M,Mathe K,Töröcsik B,Doczi J,Turiak L,Kiss G,Konràd C,Vajda S,Vereczki V,Oh RJ,Adam-Vizi V

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: A-SCS; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta; renal carcinoma antigen NY-REN-39; RP11-528D24.2; SCS-betaA; SCSbetaA; succinate--CoA ligase (ADP-forming); Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial; succinate-CoA ligase beta subunit; succinate-CoA ligase, ADP-forming, beta subunit; succinate-Coenzyme A ligase, ADP-forming, beta subunit; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; Succinyl-CoA synthetase beta-A chain

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Gene Aliases: 4930547K18Rik; A-BETA; MTDPS5; SCS-betaA; SUCLA2

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UniProt ID: (Human) Q9P2R7, (Mouse) Q9Z2I9

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Entrez Gene ID: (Human) 8803, (Rat) 361071, (Mouse) 20916

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Function(s)

Process(es)

tricarboxylic acid cycle succinyl-CoA metabolic process succinate metabolic process succinyl-CoA pathway metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SUCLA2 Polyclonal Antibody

SUCLA2 Antibody (12627-1-AP) in ICC/IF

Product Details

12627-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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