Invitrogen

SUFU Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (16) SUFU antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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SUFU Antibody in Western Blot (WB) — Western blot analysis of Suppressor of Fused using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a dilution of 1:1000.

SUFU Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Suppressor of Fused in the cytosol and nucleus of paraffin-embedded mouse middle brain using a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a dilution of 1:500.

SUFU Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Suppressor of Fused in paraformaldehyde-fixed HeLa cells using a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a 1:200 dilution.

SUFU Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of SUFU was observed in Mouse adult Kidney and Mouse adult Brain in comparison to Mouse Pup Kidney and Mouse Post-natal (Day1) Brain using SUFU Polyclonal Antibody (Product # PA5-29952) in western blot. . {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunocytochemistry (ICC/IF)

1:100-1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 267 and 484 of Human Suppressor of Human Fused

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547426

Product Specific Information

Recommended positive controls: A431, H1299, HeLa, HepG2.

Predicted reactivity: Mouse (97%), Rat (95%), Xenopus laevis (89%), Chicken (95%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.