Invitrogen

SUFU Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (12) SUFU antibodies

Datasheet

Datasheet

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SUFU Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Suppressor of Fused in paraformaldehyde-fixed HeLa cells using a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a 1:200 dilution.

SUFU Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Suppressor of Fused in the cytosol and nucleus of paraffin-embedded mouse testis tissue using a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a dilution of 1:500.

SUFU Antibody in Western Blot (WB) — Western blot was performed using Anti-SUFU Polyclonal Antibody (Product # PA5-29952) and a 43kDa band corresponding to SUFU was observed in Mouse Adult Kidney and Mouse Adult Brain, and not in Mouse Pup Kidney and Mouse Post-natal (Day1) Brain, which are reported negative for SUFU expression. Tissue extracts (30 µg lysate) of Mouse Pup Kidney (Lane 1), Mouse adult Kidney (Lane 2), Mouse Post-natal (Day1) Brain (Lane 3) and Mouse adult Brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

SUFU Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of SUFU was observed in Mouse adult Kidney and Mouse adult Brain in comparison to Mouse Pup Kidney and Mouse Post-natal (Day1) Brain using SUFU Polyclonal Antibody (Product # PA5-29952) in western blot. . {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 267 and 484 of Human Suppressor of Human Fused

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547426

Product Specific Information

PA5-29952 targets Suppressor of Fused in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-29952 immunogen is recombinant fragment corresponding to a region within amino acids 267 and 484 of Human Suppressor of Human Fused.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.