Molecular weight marker (lane 1). PA5-19418 staining of HeLa nuclear lysate at a dilution of 1 ug/ml (Lane 2). PA5-19418 staining of NIH 3T3 whole cell lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Hamster, Human, Mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Sumo 2+3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:800|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with rat, cow, pig and zebrafish based on sequence homology.
SUMO proteins, such as SUMO3, and ubiquitin posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.