Invitrogen

SUPV3L1 Polyclonal Antibody

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SUPV3L1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of SUPV3L1 was performed in NIH-3T3 cells using SUPV3L1 Polyclonal Antibody (Product # PA5-97306) at a dilution of 1:100. Blue: DAPI for nuclear staining.

SUPV3L1 Antibody in Western Blot (WB) — Western Blot analysis of SUPV3L1 in extracts of various cell lines using SUPV3L1 Polyclonal Antibody (Product # PA5-97306) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-97306

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 577-786 of human SUPV3L1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.07 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2809108

Product Specific Information

Immunogen sequence: APINKKQPFV CSSLLQFARQ YSRNEPLTFA WLRRYIKWPL LPPKNIKDLM DLEAVHDVLD LYLWLSYRFM DMFPDASLIR DLQKELDGII QDGVHNITKL IKMSETHKLL NLEGFPSGSQ SRLSGTLKSQ ARRTRGTKAL GSKATEPPSP DAGELSLASR LVQQGLLTPD MLKQLEKEWM TQQTEHNKEK TESGTHPKGT RRKKKEPDSD; Positive Samples: K-562, Raji, Jurkat, SW620, Mouse heart, Mouse kidney, Mouse liver; Cellular Location: Mitochondrion matrix, Nucleus, mitochondrion nucleoid

Target Information

Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed:29967381). ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase SUPV3L1, mitochondrial; Suppressor of var1 3-like protein 1; suppressor of var1, 3-like 1; suppressor of var1, 3-like 1(SUV3); SUV3-like helicase; SUV3-like protein 1

Gene Aliases: 6330443E10Rik; SUPV3L1; SUV3; Suv3l1

UniProt ID: (Human) Q8IYB8, (Mouse) Q80YD1, (Rat) Q5EBA1

Entrez Gene ID: (Human) 6832, (Mouse) 338359, (Rat) 294385

Molecular Function: DNA metabolism protein