|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal portion (within residues 1400-1500) of human Separase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||citric acid, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Suggested positive control: A431 whole cell extracts.
Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell.
Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.
PA1-46455 was used in western blot to study the effect of imatinib on separase proteolytic activity in BCR-ABL-positive and -negative cells
|Haaß W,Stehle M,Nittka S,Giehl M,Schrotz-King P,Fabarius A,Hofmann WK,Seifarth W||PloS one (7:null)||2012|