Invitrogen

Septin 2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (22) Septin 2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Septin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Septin 2 in human cell line U-2 OS shows positivity in nucleus, nucleoli & actin filaments. Samples were probed using a Septin 2 Polyclonal Antibody (Product # PA5-53814).

Septin 2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Septin 2 in human gallbladder using a Septin 2 Polyclonal Antibody (Product # PA5-53814) shows moderate cytoplasmic positivity in glandular cells.

Septin 2 Antibody in Western Blot (WB) — Western blot analysis of Septin 2 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a Septin 2 Polyclonal Antibody (Product # PA5-53814). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Septin 2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251 mg cells were transfected with, control siRNA, Septin 2 siRNA probe #1, and Septin 2 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-Septin 2 Polyclonal Antibody (Product # PA5-53814) used at a dilution of 0.4 µg/mL. Anti-PPIB was used as a loading control. {KD}

Product Details

PA5-53814

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human Septin 2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2647124

Product Specific Information

Immunogen sequence: WGVVEVENPE HNDFLKLRTM LITHMQDLQE VTQDLHYENF RSERLKRGGR KVENEDMNKD QILLEKEAEL RRMQEMIARM QAQMQMQMQG GDGDGGALGH

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 96%.

Target Information

Septin 2 is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton and plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. It is required for the progression through mitosis, and it forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, Septin 2 may be required for chromosome segregation and spindle elongation. Septin 2 also plays a role in ciliogenesis and collective cell movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2-Sep; NEDD-5; Neural precursor cell expressed developmentally down-regulated protein 5; neural precursor cell expressed, developmentally down-regulated 5; neural precursor cell expressed, developmentally down-regulated gene 5; Septin-2; Vascular endothelial cell specific protein 11

Gene Aliases: AW208991; DIFF6; hNedd5; KIAA0158; mKIAA0158; NEDD-5; NEDD5; Pnutl3; SEPT2; SEPTIN2; Vesp11

UniProt ID: (Human) Q15019, (Mouse) P42208, (Rat) Q91Y81

Entrez Gene ID: (Human) 4735, (Mouse) 18000, (Rat) 117515

Molecular Function: cytoskeletal protein