Invitrogen

Septin 2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (11) Septin 2 antibodies

Datasheet

Datasheet

Product Image Gallery

Septin 2 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of Septin 2 in human cell line U-2 OS shows positivity in nucleus, nucleoli and actin filaments. Samples were probed using a Septin 2 Polyclonal Antibody (Product # PA5-53814).

Septin 2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Septin 2 in human gallbladder using a Septin 2 Polyclonal Antibody (Product # PA5-53814) shows moderate cytoplasmic positivity in glandular cells.

Septin 2 Antibody in Western Blot (WB) — Western blot analysis of Septin 2 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a Septin 2 Polyclonal Antibody (Product # PA5-53814). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Septin 2 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251MG cells were transfected with, control siRNA, Septin 2 siRNA probe #1, and Septin 2 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-Septin 2 Polyclonal Antibody (Product # PA5-53814) used at a dilution of 0.4 µg/ml. Anti-PPIB was used as a loading control. {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Septin 2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2647124

Product Specific Information

Immunogen sequence: WGVVEVENPE HNDFLKLRTM LITHMQDLQE VTQDLHYENF RSERLKRGGR KVENEDMNKD QILLEKEAEL RRMQEMIARM QAQMQMQMQG GDGDGGALGH

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 96%.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: developmentally down-regulated 5; DIFF6; hNedd5; NEDD-5; NEDD5; neural precursor cell expressed; Neural precursor cell expressed developmentally down-regulated protein 5; neural precursor cell expressed, developmentally down-regulated 5; neural precursor cell expressed, developmentally down-regulated gene 5; Pnutl3; Septin-2; Vascular endothelial cell specific protein 11

Gene Aliases: AW208991; DIFF6; hNedd5; KIAA0158; mKIAA0158; NEDD-5; NEDD5; Pnutl3; SEPT2; Vesp11

UniProt ID: (Human) Q15019, (Mouse) P42208, (Rat) Q91Y81

Entrez Gene ID: (Human) 4735, (Mouse) 18000, (Rat) 117515

Molecular Function: G-protein cytoskeletal protein enzyme modulator small GTPase