Proteintech

Sestrin 2 Polyclonal Antibody

View all (14) SESN2 antibodies

Questions & Answers

Questions & Answers

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Sestrin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SESN2 antibody 10795-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

Sestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:50 (under 25x lens).

Sestrin 2 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

Sestrin 2 Antibody in Western Blot (WB) — A2780 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Sestrin 2 Antibody in Western Blot (WB) — A549 cells (shcontrol and shRNA of SESN2) were subjected to SDS PAGE followed by western blot with 10795-1-AP (SESN2 antibody) at dilution of 1:1000.

Sestrin 2 Antibody in Western Blot (WB) — WB result of Sestrin antibody (10795-1-AP, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.

Sestrin 2 Antibody in Immunoprecipitation (IP) — IP result of anti-Sestrin 2 (IP:10795-1-AP, 3ug; Detection:10795-1-AP 1:1000) with K-562 cells lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 80 publications 80 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Rat, Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sestrin 2 Fusion Protein Ag1247

Conjugate

Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hypoxia induced gene 95; hypoxia-induced; Hypoxia-induced gene; Sestrin-2

Gene Aliases: HI95; RGD1566319; SES2; SESN2; SEST2

UniProt ID: (Human) P58004, (Mouse) P58043

Entrez Gene ID: (Human) 83667, (Rat) 502988, (Mouse) 230784

Molecular Function: peroxidase

regulation of protein phosphorylation fatty acid beta-oxidation autophagy response to glucose positive regulation of macroautophagy negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator mitochondrial DNA metabolic process response to insulin cellular response to oxidative stress positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress protein kinase B signaling glucose import triglyceride homeostasis cellular response to amino acid stimulus cellular response to leucine reactive oxygen species metabolic process regulation of gluconeogenesis involved in cellular glucose homeostasis cellular oxidant detoxification positive regulation of protein localization to nucleus regulation of response to reactive oxygen species negative regulation of translation in response to endoplasmic reticulum stress negative regulation of TORC1 signaling regulation of cAMP-dependent protein kinase activity mitochondrion organization glucose homeostasis

Process(es)

protein binding oxidoreductase activity, acting on peroxide as acceptor sulfiredoxin activity leucine binding