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Sigma-1 Receptor Antibody (42-3300) in WB
Western blot of SIGMAR1 was performed by loading 30 µg of WT (lanes 1/3) and SIGMAR1 CRISPR KO (lanes 2/4) HEK293T or HeLa cell lysates in RIPA buffer onto a 5-16% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. SIGMAR1 was detected at approximately 25 kDa (designated by the black arrow) using a SIGMAR1 polyclonal antibody (Product # 42-3300) at a dilution of 1:5,000 in 5% BSA in TBS with 0.1% T... View More
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.jpg?time=20220826?time=20220826 "Sigma-1 Receptor Antibody (42-3300) in WB")
Product Details
42-3300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
C. elegans,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the rat sigma-1 receptor, which differs from mouse and human by one conservative amino acid change and is predicted to be identical to dog, bovine, and ermine
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the sigma-1 receptor (sigma-1R, Oprs1 protein, opioid receptor sigma 1) protein. On western blots, it identifies the target band at ~25 kDa. Reactivity has been confirmed with rat brain, rat liver, and mouse brain homogenates. Based on amino acid sequence identity, reactivity with human, bovine, dog, and ermine is expected.
Target Information
This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AL024364; mSigmaR1; opioid receptor, sigma 1; RP23 167I12.6; SIG 1R; sigma 1 receptor variant SR-1D; Sigma 1-type opioid receptor; Sigma non-opioid intracellular receptor 1; Sigma-1 Receptor; sigma1 receptor; Sigma1-receptor; sigma1R; SR BP
Gene Aliases: Oprs1; sigma1R; Sigmar1
UniProt ID: (Rat) Q9R0C9, (Mouse) O55242
Entrez Gene ID: (Rat) 29336, (Mouse) 18391
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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