|Tested species reactivity||Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the carboxy terminus of mouse SirT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other sirtuin proteins.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor ¿ in MC3T3-E1 cells.
PA5-17074 was used in immunoprecipitation and western blot to investigate the molecular mechanism of Sirt1 in osteogenic differentiation
|Qu B,Ma Y,Yan M,Gong K,Liang F,Deng S,Jiang K,Ma Z,Pan X||Biochemical and biophysical research communications (478:439)||2016|
MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor ß/¿-dependent manner.
PA5-17074 was used in western blot to elucidate mechanisms by which miR-132 regulates osteogenic differentiation
|Gong K,Qu B,Liao D,Liu D,Wang C,Zhou J,Pan X||Biochemical and biophysical research communications (478:260)||2016|