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MUbio
Smoothelin Monoclonal Antibody (R4A)
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Product Details
MUB1700P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Cat,
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
R4A
Immunogen
Smoothelin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
r4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ35365; FLJ38597; Smoothelin; smoothelin-B; SMTN
Gene Aliases: SMSMO; SMTN
UniProt ID: (Human) P53814
Entrez Gene ID: (Human) 6525, (Chicken) 100858894, (Pig) 414369, (Dog) 477542
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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