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Invitrogen
Smoothelin Monoclonal Antibody (R4A)
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Product Details
OMA1-06020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Cat,
Human,
Mouse,
Non-human primate,
Pig
Published species
Human,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
R4A
Immunogen
Cytoskeletal smoothelin extract of chicken gizzard.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken and monkey tissues. This antibody detects both the visceral and vascular smoothelin isoforms. Does not cross react with rat samples.
OMA1-06020 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~59 kDa protein representing visceral smoothelin and an ~100 kDa protein representing vascular smoothelin from canine smooth muscle extracts. This antibody can be used to discriminate fully differentiated (contractile) smooth muscle cells from proliferative smooth muscle cells. Staining of formalin-fixed paraffin-embedded tissues requires boiling in 10 mM citrate buffer (pH 6.0) for 3x5 min, prior to immunostaining.
OMA1-06020 antigen is cytoskeletal smoothelin extract of chicken gizzard.
Store at 4°C, or in small aliquots at -20°C.
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ35365; FLJ38597; Smoothelin; smoothelin-B; SMTN; unnamed protein product
Gene Aliases: SMSMO; SMTN
UniProt ID: (Human) P53814, (Mouse) Q921U8
Entrez Gene ID: (Human) 6525, (Chicken) 100858894, (Pig) 414369, (Dog) 477542, (Mouse) 29856
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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