Invitrogen

Sorcin Polyclonal Antibody

View all (11) Sorcin antibodies

Questions & Answers

Questions & Answers

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Sorcin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Sorcin in formalin-fixed paraffin-embedded tissue section of human small intestine. Samples were incubated in Sorcin polyclonal antibody (Product # PA5-23143) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. Cytosplasmic staing of the glandular cells was observed.

Sorcin Antibody in Western Blot (WB) — Western blot analysis of Sorcin in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain. Samples were incubated in Sorcin polyclonal antibody (Product # PA5-23143).

Product Details

PA5-23143

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	2-5 µg/mL	-

Product Specifications
Species Reactivity	Bovine, Dog, Horse, Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide (60 QSGIAGGYKPFNLET 74) of Sorcin protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2540670

Target Information

Sorcin is a 198 amino acid, soluble, resistance-related, calcium-binding cytosolic protein belonging to the penta-EF hand (PEF) family of calcium binding proteins and contains 4 EF-hand calcium-binding motifs. Human Sorcin is known to share 95% sequence identity with hamster Sorcin. It is known to associate with the sarcolemmal proteins Annexin VII, cardiac ryanodine receptors and L-type Ca2+ channels and thus influence the intracellular Ca (2+)-homeostasis. It is also important in the regulation of intracellular Ca2+ cycling and Ca2+ influx pathways in the heart and skeletal muscle during excitation-contraction. It is overproduced in many multi-drug resistance (MDR) cells and regulates cell apoptosis pathways, thus acting as a new MDR marker for prognosis of acute myeloid leukemia (AML) and a good target for anti-MDR drug development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanisms of beneficial effects of metformin on fatty acid-treated human islets

Journal of molecular endocrinology

Cen J,Sargsyan E,Forslund A,Bergsten P

PA5-23143 was used in Western Blotting to study the effect of metforminon FFA-treated human islets and the mechanisms underlying these effects.

Mon Oct 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells; CP-22; CP22; H_RG167B05.1; Sorcin; V19

Gene Aliases: 2210417O06Rik; 2900070H08Rik; CP-22; CP22; SCN; Sor; SRI; V19

UniProt ID: (Human) P30626, (Mouse) Q6P069

Entrez Gene ID: (Human) 6717, (Bovine) 508319, (Horse) 100059619, (Dog) 475220, (Mouse) 109552, (Rat) 683667

Molecular Function: actin or actin-binding cytoskeletal protein

action potential proteolysis transport calcium ion transport intracellular sequestering of iron ion regulation of striated muscle contraction signal transduction heart development muscle organ development regulation of heart contraction negative regulation of heart rate regulation of cell communication by electrical coupling regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum positive regulation of insulin secretion involved in cellular response to glucose stimulus cytoplasmic sequestering of transcription factor positive regulation of release of sequestered calcium ion into cytosol regulation of calcium ion transport negative regulation of ryanodine-sensitive calcium-release channel activity regulation of cardiac muscle cell contraction regulation of relaxation of muscle regulation of high voltage-gated calcium channel activity regulation of cell communication by electrical coupling involved in cardiac conduction negative regulation of transcription regulatory region DNA binding

Process(es)

protease binding calcium-dependent cysteine-type endopeptidase activity receptor binding calcium channel regulator activity calcium ion binding protein binding ion channel binding protein heterodimerization activity repressing transcription factor binding metal ion binding