|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from residues 374 - 423 of human sP7/Osterix.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 2% sucrose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/ml|
|Western Blot (WB)||0.125 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30865 detects Sp7 from human samples.
PA1-30865 has been successfully used in immunohistochemistry (paraffin tissue) and Western blot applications. Fetal kidney lysate can serve as a positive control for Western blot; Human intestinal tissue can be used for a positive control for immunohistochemical applications.
The PA1-30865 immunogen is a synthetic peptide derived from residues 374 - 423 of human sP7/Osterix.
SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.