Western blot analysis of HeLa Whole Cell Lysate using PA5-19604, Spt6 primary antibody at a dilution of 1:500 (lane 1). PA5-19604 staining of Jurkat Whole Cell Lysate at a dilution of 1:500 (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Human Spt6.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:30|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with mouse and zebrafish based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.