Immunohistochemical analysis of Stomatin in paraffin-embedded human liver following steamed antigen retrieval with a citrate buffer, pH 6, using a Stomatin polyclonal antibody (Product# PA5-37857) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence EKRHTRDSEAQR-C, from the N-terminus of STOM|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.1-0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.