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          • Primary Antibodies ›
          • Syk Antibodies

          Invitrogen

          Syk Polyclonal Antibody

          Advanced Verification
          1 Reference
          View all (54) Syk antibodies

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          Cite Syk Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          • Advanced Verification (2)
          Syk Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Syk Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Syk Antibody (PA5-96063) in ICC/IF

          Immunofluorescence analysis of Tyrosine-protein kinase SYK was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Syk Polyclonal Antibody (Product # PA5-96063) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Syk Antibody in Immunocytochemistry (ICC/IF)
          Syk Antibody in Immunocytochemistry (ICC/IF)
          Syk Antibody in Western Blot (WB)
          Syk Antibody in Western Blot (WB)
          Syk Antibody in Western Blot (WB)
          Syk Antibody
          Syk Antibody

          Product Details

          PA5-96063

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.32 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807865

          Product Specific Information

          Positive Samples: HL-60, Mouse heart; Cellular Location: Cell membrane, Cytoplasm, cytosol

          Target Information

          Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; p72Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK; unnamed protein product

          View more View less

          Gene Aliases: IMD82; p72-Syk; p72syk; ptk72; SYK; Sykb

          View more View less

          UniProt ID: (Human) P43405, (Rat) Q64725, (Mouse) P48025

          View more View less

          Entrez Gene ID: (Human) 6850, (Rat) 25155, (Mouse) 20963

          View more View less

          Function(s)
          nucleotide binding phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding interleukin-15 receptor binding kinase activity transferase activity protein kinase binding phosphatase binding Toll-like receptor binding SH2 domain binding phospholipase binding scaffold protein binding receptor signaling protein tyrosine kinase activity protein domain specific binding transferase activity, transferring phosphorus-containing groups kinase modulator
          Process(es)
          autophagosome assembly angiogenesis cell activation positive regulation of cytokine production serotonin secretion lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response immune system process serotonin secretion by platelet cell surface pattern recognition receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus protein phosphorylation protein import into nucleus leukocyte cell-cell adhesion cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway integrin-mediated signaling pathway organ morphogenesis negative regulation of autophagy positive regulation of autophagy regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway peptidyl-tyrosine phosphorylation leukotriene biosynthetic process calcium-mediated signaling platelet activation B cell differentiation neutrophil chemotaxis positive regulation of protein complex assembly receptor internalization cellular response to nutrient levels positive regulation of type I interferon production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-3 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of mast cell cytokine production regulation of superoxide anion generation positive regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin intracellular signal transduction collagen-activated tyrosine kinase receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis interleukin-3-mediated signaling pathway TORC1 signaling gamma-delta T cell differentiation defense response to bacterium mast cell degranulation positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection positive regulation of MAPK cascade innate immune response positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis regulation of phagocytosis regulation of immune response positive regulation of calcium-mediated signaling B cell receptor signaling pathway leukocyte migration regulation of sequence-specific DNA binding transcription factor activity establishment of localization in cell regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to lipid cellular response to low-density lipoprotein particle stimulus positive regulation of monocyte chemotactic protein-1 production regulation of arachidonic acid secretion regulation of platelet aggregation apoptotic signaling pathway beta-amyloid clearance positive regulation of cold-induced thermogenesis negative regulation of TORC1 signaling positive regulation of TORC1 signaling cellular response to beta-amyloid cellular response to lectin activation of MAPK activity inflammatory response transmembrane receptor protein tyrosine kinase signaling pathway activation of JUN kinase activity cell migration peptidyl-serine phosphorylation signal transduction by protein phosphorylation peptidyl-tyrosine autophosphorylation transcription factor import into nucleus positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process protein autophosphorylation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation G-protein coupled receptor signaling pathway phosphorylation cell differentiation
          It has to be done as per old AB suggested Products section.

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