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Peripheral neurons in mouse intestinal cryosections were labeled with rabbit anti–synapsin I antibody (Cat. No. A6442) and detected using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). This tissue was counterstained with DAPI (Cat. No. D1306, D3571, D21490).
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified bovine brain Synapsin 1|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:5000|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody has little or no activity against Synapsin II.
Storage and reconstitution: when stored at -20°C or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4°C, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20°C, avoiding freeze/thaw cycles.
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system.
A-6442 was used in immunohistochemistry to identify a method for simultaneous use of both biochemistry and immunohistochemistry in regards to the same tissue
|Notter T,Panzanelli P,Pfister S,Mircsof D,Fritschy JM||The European journal of neuroscience (39:165)||2014|
An in vitro model of developmental synaptogenesis using cocultures of human neural progenitors and cochlear explants.
A-6442 was used in immunocytochemistry to characterize differentiated hESC-derived neural progenitors.
|Nayagam BA,Edge AS,Needham K,Hyakumura T,Leung J,Nayagam DA,Dottori M||Stem cells and development (22:901)||2013|
Regional differentiation of retinoic acid-induced human pluripotent embryonic carcinoma stem cell neurons.
A-6442 was used in immunocytochemistry to identify the transcription factors that determine the rostocaudal and dorsoventral identity of NT2N derived from a monolayer differentiation model.
|Coyle DE,Li J,Baccei M||PloS one (6:null)||2011|
Proteomics analysis reveals overlapping functions of clustered protocadherins.
A-6442 was used in western blot to delineate the composition of PCDH-gamma complexes and its role in neural development.
|Han MH,Lin C,Meng S,Wang X||Molecular & cellular proteomics : MCP (9:71)||2010|
Brain protein 4.1; synapsin 1; Synapsin I; Synapsin-1; truncated synapsin 1-S
BOS_25913; Syn-1; SYN1; Syn1-S; SYN1a; SYN1b; SYNI