|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native protein purified from bovine brain|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, this antibody detects an ~77 kDa and ~80 kDa protein representing synapsin I alpha and synapsin I beta respectively, in rat brain homogenate. Immunohistochemical staining of synapsin 1 alpha and 1 beta in human brain with OPA1-04001 yields a pattern consistent with cytoplasmic vesicle staining.
There are four synapsin protein subtypes that are encoded by two synapsin genes. Alternate splicing of synapsin I and II transcripts results in the production of synapsin subtypes I alpha, Ib eta, II alpha, and II beta. These proteins share homology in their amino-terminal and central domains. CaM Kinase I and cAMP-dependent protein kinase phosphorylation sites are present within the central domain of synapsin I and II. Phosphorylation sites for CaM Kinase II are found within the carboxy-terminal domains of synapsin I alpha and I beta only.
Synapsin Ialpha and Ibeta are similar neuron-specific peripheral membrane proteins that localize to the cytoplasmic face of small synaptic vesicles. Synapsin Il alpha and I beta have been implicated in the clustering of these vesicles at release sites prior to exocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.