|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native protein purified from bovine brain|
|Storage buffer||5mM Ammonium bicarbonate, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||1ug per 200 ug lysate|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for the ~78 kDa Synapsin I protein doublet. Immunolabeling is blocked by preadsorption of the antibody with the protein used to generate the antibody.
Reconstitute with 50 ul of PBS.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovi et al., 2001; Kao et al., 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.