|Tested species reactivity||Bovine, Mouse, Sheep, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1043 detects Synaptophysin in rat, mouse, bovine, and sheep samples.
PA1-1043 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~33.8 kDa protein representing Synaptophysin in rat, mouse and bovine brain tissue as well as sheep retinas.
The PA1-1043 immunogen is a synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin. This sequence is conserved in many species including human, mouse and bovine. The PA1-1043 immunizing peptide (Cat. # PEP-279) is available for use in neutralization and control experiments.
Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis.
Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enhanced hippocampal long-term potentiation and fear memory in Btbd9 mutant mice.
PA1-1043 was used in western blot to study the effects of restless leg syndrome-associated Btbd9 mutant on pre-synaptic activity, long-term hippocampal potentiation and fear memory in a murine model
|DeAndrade MP,Zhang L,Doroodchi A,Yokoi F,Cheetham CC,Chen HX,Roper SN,Sweatt JD,Li Y||PloS one (7:null)||2012|
Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce.
PA1-1043 was used in western blot to investigate the Dyt1 and Sgce mutations in motor deficits
|Yokoi F,Yang G,Li J,DeAndrade MP,Zhou T,Li Y||Journal of biochemistry (148:459)||2010|
Wnt-7a modulates the synaptic vesicle cycle and synaptic transmission in hippocampal neurons.
PA1-1043 was used in immunocytochemistry to study the role of Wnt-7a in modulating hippocampal neuron presynaptic function
|Cerpa W,Godoy JA,Alfaro I,Farías GG,Metcalfe MJ,Fuentealba R,Bonansco C,Inestrosa NC||The Journal of biological chemistry (283:5918)||2008|