Immunofluorescence analysis of Syndecan-2 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Syndecan-2 Rabbit polyclonal Antibody (366200) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mammal, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Syndecans are heparan sulfate proteoglycans (HSPGs) that are involved in cell adhesion, tissue morphogenesis, differentiation, and act as low affinity co-receptors of soluble growth factors. It is involved in the mitogenic activity and signaling of Granulocyte-Macrophage Colony-stimulating factor in osteoblasts, and has been shown to have a regulatory role in matrix assembly. Syndecan-2 is expressed at epithelial-mesenchymal interfaces, and prechondrogenic and preosteogenic mesenchymal condensations, which are areas of high morphogenetic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Syndecan-2 is a key regulator of transforming growth factor beta 2/Smad2-mediated adhesion in fibrosarcoma cells.
36-6200 was used in western blot to test if syndecan-2 affects TGFβ2 functions.
|Mytilinaiou M,Bano A,Nikitovic D,Berdiaki A,Voudouri K,Kalogeraki A,Karamanos NK,Tzanakakis GN||IUBMB life (65:134)||2013|
|CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.||Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A||Molecular biology of the cell (15:5635)||2004|
Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery.
36-6200 was used in immunocytochemistry to determine if pregnancy alters proteoglycan production in cervical fibroblasts
|Akerud A,Dubicke A,Sennstrom M,Ekman-Ordeberg G,Malmstrom A||Acta obstetricia et gynecologica Scandinavica (87:1220)||2008|