Immunohistochemistry analysis of Syndecan-4 showing staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-4 polyclonal antibody (363100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Mammal, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the middle region of the human Syndecan-4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Syndecan-4 is a transmembrane (type I) heparan sulfate proteoglycan containing 198 amino acids and belonging to the syndecan proteoglycan family. Syndecan-4 functions as a receptor or coreceptor in intracellular signaling. Initially, Syndecan-4 was isolated as ryudocan, from rat endothelial cells, and from human epithelial and fibroblastic cells, as amphiglycan. The syndecan heparan sulfate chains of Syndecan-4 binds to HIV-1 gp120 and thus functions as trans HIV receptors. This cell surface proteoglycan usually exists as a homodimer that interacts with CDCP1, with GIPC PDZ domain and with NUDT16L1/SDOS. Reports suggest that Syndecan-4 is often upregulated in skin dermis after wounding, thus playing an important role in wound healing and angiogenesis. It is also needed in satellite cell function and in the development and regeneration of skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||1:50||Syndecan-4 knockout leads to reduced extracellular transglutaminase-2 and protects against tubulointerstitial fibrosis.||Scarpellini A,Huang L,Burhan I,Schroeder N,Funck M,Johnson TS,Verderio EA||Journal of the American Society of Nephrology : JASN (25:1013)||2014|
|Human||Not Cited||Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.||Kalia M,Chandra V,Rahman SA,Sehgal D,Jameel S||Journal of virology (83:12714)||2009|
|Human||Not Cited||Hypoxia increases macrophage motility, possibly by decreasing the heparan sulfate proteoglycan biosynthesis.||Asplund A,Ostergren-Lundén G,Camejo G,Stillemark-Billton P,Bondjers G||Journal of leukocyte biology (86:381)||2009|
|CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.||Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A||Molecular biology of the cell (15:5635)||2004|