|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide surrounding amino acid 184 of human Syndecan 4.|
|Storage buffer||PBS, pH 7.2, with 1% BSA, 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||15-25 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||15-25 µg/ml|
|Immunoprecipitation (IP)||5-10 µg/ml|
|Western Blot (WB)||1-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32485 detects Syndecan 4 from mouse, rat, human samples.
PA1-32485 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot, immunoprecipitation applications. Western blot detects a band of approximately 24kDa (predicted molecular weight: 22kDa.
The PA1-32485 immunogen is synthetic peptide surrounding amino acid 184 of human Syndecan 4.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Syndecans are tyupe I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan 1 is a matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan 1 and Syndecan 3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan 2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesencymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan 4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.