|Immunohistochemistry (Frozen) (IHC (F))||15-25 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||15-25 µg/ml|
|Immunoprecipitation (IP)||5-10 µg/ml|
|Western Blot (WB)||1-4 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide surrounding amino acid 184 of human Syndecan 4.|
|Storage buffer||PBS, pH 7.2, with 1% BSA, 50% glycerol|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-32485 detects Syndecan 4 from mouse, rat, human samples.
PA1-32485 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot, immunoprecipitation applications. Western blot detects a band of approximately 24kDa (predicted molecular weight: 22kDa.
The PA1-32485 immunogen is synthetic peptide surrounding amino acid 184 of human Syndecan 4.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Syndecans are tyupe I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan 1 is a matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan 1 and Syndecan 3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan 2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesencymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan 4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Amphiglycan; MGC22217; ryudocan amphiglycan; Ryudocan core protein; Ryudocan/syndecan 4; SYND4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4
Gene Aliases: AA959608; AW108331; RATRYUDOCA; RYUDOCA; ryudocan; S4; SDC4; SYND4; syndecan-4
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