Immunofluorescence analysis of Syntaxin 1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Syntaxin-1 Rabbit Polyclonal Antibody (PA11042) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues V(22) T V D R D R F M D E F F E Q V E E I R(41) of rat Syntaxin 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-1042 detects Syntaxin 1 in rat, mouse and bovine samples.
PA1-1042 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~33 kDa protein representing Syntaxin 1 in rat, mouse and bovine brain tissue samples.
The PA1-1042 immunogen is a synthetic peptide corresponding to residues V(22) T V D R D R F M D E F F E Q V E E I R(41) of rat Syntaxin 1. This sequence is conserved in mouse, human and bovine. The PA1-1042 immunizing peptide (Cat. # PEP-278) is available for use in neutralization and control experiments.
Syntaxin 1 is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells and thought to be implicated in the regulation of synaptic neurotransmitter release.
Syntaxin 1 interacts with several other proteins important for synaptic function (SNAP 25, Munc 18). Syntaxin 1 is the main target of one of the Botulinum neurotoxins (BoNT/C1) which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.
Syntaxin 1 is an essential component of the neurotransmitter release machinery, and regulation of Syntaxin 1 expression levels is thought to contribute to the mechanism underlying learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enhanced hippocampal long-term potentiation and fear memory in Btbd9 mutant mice.
PA1-1042 was used in western blot to study the effects of restless leg syndrome-associated Btbd9 mutant on pre-synaptic activity, long-term hippocampal potentiation and fear memory in a murine model
|DeAndrade MP,Zhang L,Doroodchi A,Yokoi F,Cheetham CC,Chen HX,Roper SN,Sweatt JD,Li Y||PloS one (7:null)||2012|