Invitrogen

Syntaxin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (26) Syntaxin 1 antibodies

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Datasheet

Protocols

Tech Support

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Syntaxin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Syntaxin 1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Syntaxin-1 Rabbit Polyclonal Antibody (Product # PA1-1042) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Syntaxin 1 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue and whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2) and NCCIT (Lane 3). The blots were probed with Anti-Syntaxin 1 Rabbit Polyclonal Antibody (Product # PA1-1042, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:2500 dilution). A ~ 33 kDa band corresponding to Syntaxin 1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Syntaxin 1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of Syntaxin 1 was observed in Mouse Brain, Rat Brain, Rat Liver and Rat Kidney in Western Blot using Syntaxin 1 Polyclonal Antibody (Product # PA1-1042). Syntaxin 1 is reported to be expressed in brain tissue and not in other tissues like liver and kidney. {RE}

Product Details

PA1-1042

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2 µg/mL	-

Product Specifications
Species Reactivity	Bovine, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues V(22) T V D R D R F M D E F F E Q V E E I R(41) of rat Syntaxin 1.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2271320

Product Specific Information

PA1-1042 detects Syntaxin 1 in rat, mouse and bovine samples.

PA1-1042 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~33 kDa protein representing Syntaxin 1 in rat, mouse and bovine brain tissue samples.

The PA1-1042 immunogen is a synthetic peptide corresponding to residues V(22) T V D R D R F M D E F F E Q V E E I R(41) of rat Syntaxin 1. This sequence is conserved in mouse, human and bovine. The PA1-1042 immunizing peptide (Cat. # PEP-278) is available for use in neutralization and control experiments.

Target Information

Syntaxin 1 is a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhanced hippocampal long-term potentiation and fear memory in Btbd9 mutant mice.

PloS one

DeAndrade MP,Zhang L,Doroodchi A,Yokoi F,Cheetham CC,Chen HX,Roper SN,Sweatt JD,Li Y

PA1-1042 was used in western blot to study the effects of restless leg syndrome-associated Btbd9 mutant on pre-synaptic activity, long-term hippocampal potentiation and fear memory in a murine model

Fri Aug 31 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Neuron-specific antigen HPC-1; P35A; STX1B; STX1B1; STX1B2; STXA1; Synaptotagmin-associated 35 kDa protein; syntaxin 1 a; syntaxin 1A (brain); syntaxin 1A (brain)-like; Syntaxin-1A

Gene Aliases: Sap; STX1A

Syntaxin 1 Polyclonal Antibody

Product Details

PA1-1042

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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