Invitrogen

Syntaxin 6 Recombinant Polyclonal Antibody (8HCLC)

Product Image Gallery

Syntaxin 6 Antibody in Immunofluorescence (IF) — Immunofluorescence was performed on fixed and permeabilized MCF-7 cells for detection of Syntaxin-6 using Anti-Syntaxin-6 Recombinant Rabbit Polyclonal Antibody (Product # 710855, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Syntaxin-6 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Syntaxin-6. Panel e) represents control cells with no primary Antibody to assess background.

Syntaxin 6 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane1), U-87MG (Lane 2), SK-N-SH (Lane 3) and MDCK (Lane 4). The blots were probed with Anti-Syntaxin 6 Recombinant Rabbit Polyclonal Antibody (Product # 710855, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 30 kDa band corresponding to Syntaxin 6 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Syntaxin 6 Antibody in Immunoprecipitation (IP) — Syntaxin 6 was immunoprecipitated using 2 µg of the Syntaxin 6 Recombinant Rabbit Polyclonal Antibody (Product # 710855) from SK-N-SH cell extracts (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit (Product # 10006D). Normal Rabbit IgG was used as a negative IP control (Lane 2). 10% input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using Syntaxin 6 Recombinant Rabbit Polyclonal Antibody (Product # 710855) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1 µg/mL

Immunofluorescence (IF)

1 µg/mL

Immunoprecipitation (IP)

2 µg/mL

Western Blot (WB)

0.5-1 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Recombinant Polyclonal

Type

Antibody

Clone

8HCLC

Immunogen

Multiple peptides corresponding to Human Syntaxin6 (aa 2-234)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532774

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Bovine, Rat and Mouse.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Syntaxin 6 is a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. Syntaxin is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.