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OriGene
IDH1 Monoclonal Antibody (OTI2H9), DyLight™ 488, TrueMAB™
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FIGURE: 1 / 1
IDH1 Antibody (TA400028) in Flow
HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or IDH1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with PE-labeled IDH1 antibodies (TA400028).
Product Details
TA400028
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2H9
Immunogen
Full-length protein expressed in 293T cell transfected with human IDH1 expression vector
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 0.1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Isocitrate dehydrogenases such as IDH1 catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. This product is specific for IDH1 (mutant R132H).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; homodimer; IDH; IDH1; IDH1-mutant; IDPc; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; Oxalosuccinate decarboxylase; PICD; soluble; NADP+-dependent; unnamed protein product
Gene Aliases: HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDP; IDPC; PICD
UniProt ID: (Human) O75874
Entrez Gene ID: (Human) 3417
magnesium ion binding
isocitrate dehydrogenase (NADP+) activity
protein binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
identical protein binding
protein homodimerization activity
cadherin binding
metal ion binding
NADP binding
NAD binding
glyoxylate cycle
tricarboxylic acid cycle
isocitrate metabolic process
2-oxoglutarate metabolic process
NADP metabolic process
NADPH regeneration
glutathione metabolic process
response to oxidative stress
female gonad development
response to steroid hormone
regulation of phospholipid catabolic process
regulation of phospholipid biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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