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Invitrogen
TAF4 Polyclonal Antibody
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FIGURE: 1 / 13
TAF4 Antibody (PA5-143943) in ICC/IF
Immunocytochemistry analysis of TAF4 in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TAF4 Polyclonal antibody (Product # PA5-143943) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TAF4 recombinant protein (Position: D6-K1085).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A431 whole cell, human K562 whole cell, human Caco-2 whole cell, human U20S whole cell, human HepG2 whole cell, human PC-3 whole cell, rat brain tissue, mouse brain tissue. IHC: human ovarian serous adenocarcinoma tissue, human ovarian cancer tissue, human breast cancer tissue, human gastric adenocarcinoma tissue, human renal clear cell carcinoma tissue, human tonsil tissue, mouse brain tissue, rat brain tissue, human gastric adenocarcinoma tissue, human breast cancer tissue. ICC/IF: CACO-2 cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TAF II p105, also called TAF4B, is a celltype specific transcriptional co-activator that is a component of the TFIID complex. Expressed primarily in B cells and ovarian granulosa cells, TAF II p105 can interact with OCBA/POU2AF1 to activate B cell-specific octamerdependent transcription. Additionally, TAF II p105 plays an important role in co-activating the transcription factor NFkB and is essential for activation of anti-apoptotic genes such as TNFAIP3. TAF II p135, also known as TAF4, TAF2C, TAF2C1, TAF4A or TAFII130, is a 1,085 amino acid subunit of TFIID that accelerates transcriptional activation triggered by thyroid hormone (TR) or retinoic acid (RA). Localized to the nucleus, TAF II p135 contains one TAFH domain and is thought to bind to proteins that contain glutamine-rich domains, such as the transcription factor CREB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ41943; RNA polymerase II TBP-associated factor subunit C; TAF(II)130; TAF(II)135; TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa; TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD; TAFII 130; TAFII 135; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; Transcription initiation factor TFIID 135 kDa subunit; Transcription initiation factor TFIID subunit 4
Gene Aliases: 135kDa; AI450312; MRD73; TAF(II)130; TAF(II)135; TAF2C; TAF2C1; TAF4; TAF4A; TAFII-130; TAFII-135; TAFII130; TAFII135
UniProt ID: (Human) O00268
Entrez Gene ID: (Human) 6874, (Rat) 682097, (Mouse) 228980
core promoter sequence-specific DNA binding
DNA binding
protein binding
obsolete general RNA polymerase II transcription factor activity
aryl hydrocarbon receptor binding
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription factor activity, sequence-specific DNA binding
general transcription factor
ovarian follicle development
regulation of DNA repair
transcription, DNA-templated
DNA-templated transcription, initiation
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
mRNA transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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