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Invitrogen
TAGLN Polyclonal Antibody
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FIGURE: 1 / 11
TAGLN Antibody (PA5-27463) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-27463
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Amount
47 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, C8D30, Rat-2.
Predicted reactivity: Mouse (96%), Rat (96%), Pig (98%), Chicken (85%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 22 kDa actin-binding protein; Actin-associated protein p27; DKFZp686B01212; DKFZp686P11128; epididymis secretory sperm binding protein; Protein WS3-10; SM22; sm22 alpha; SM22-alpha; Sm22alpha; smooth muscle 22 protein; Smooth muscle protein 22-alpha; TAGLN; Transgelin; unnamed protein product
Gene Aliases: SM22; SM22-alpha; Sm22a; SMCC; TAGLN; TAGLN1; TGLN; WS3-10; Ws310
UniProt ID: (Human) Q01995, (Rat) P31232, (Mouse) P37804
Entrez Gene ID: (Human) 6876, (Rat) 25123, (Mouse) 21345
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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