Invitrogen

TAL1 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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TAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human hepatocyte tissue using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).

TAL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-TAL1 Polyclonal Antibody (Product # PA5-46840) and 40 kDa band corresponding to TAL1 was observed across the cell lines tested except HL-60, HCT 116 and OVCAR-3 which are reported to be negative. An uncharacterized band of ~ 55 kDa was observed in negative cell lines. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEL 92.1.7 (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4), HL-60 (Lane 5), HCT 116 (Lane 6), OVCAR-3 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

TAL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression of TAL1 was observed in HEL 92.1.7, K-562, Jurkat , MOLT-4 and not in HL-60, HCT 116 and OVCAR-3 using TAL1 Polyclonal Antibody (Product # PA5-46840) in Western Blot. {RE}

Product Details

PA5-46840

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5 - 0.6 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	4-8 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the C-terminal of human TAL1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609317

Product Specific Information

Peptide sequence: QDVLSPNSSC GSSLDGAASP DSYTEEPAPK HTARSLHPAM LPAADGAGPR

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%

Target Information

TAL-1 is a basic helix-loop-helix transcription. It regulates differentiation and survival during hemopoiesis. TAL-1 is also implicated in the genesis of hemopoietic malignancies and it may play an important role in hemopoietic differentiation. It serves as a positive regulator of eryhtroid differentiation. Mutations are associated with T-cell leukemia and melanoma. TAL-1 is know to bind to the LIM domain containing protein Rhombotin-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHa17; Class A basic helix-loop-helix protein 17; HGNC:11556; Stem cell protein; T-cell acute lymphocytic leukemia 1; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; TAL-1; tal-1 product

Gene Aliases: BHLHA17; SCL; tal-1; TAL1; TCL5

UniProt ID: (Human) P17542

Entrez Gene ID: (Human) 6886

Molecular Function: basic helix-loop-helix transcription factor