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TAOK2 Polyclonal Antibody

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TAOK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 21188-1-AP) (TAOK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAOK2 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TAOK2 Antibody in Immunoprecipitation (IP) — IP result of anti-TAOK2 (IP:21188-1-AP, 4ug; Detection:21188-1-AP 1:500) with HEK-293 cells lysate 3000ug.

Product Details

21188-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 3 publications 3 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TAOK2 Fusion Protein Ag15521 (742-969 aa encoded by BC142663) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

The immunogen of this antibody is 742-969 aa of human TAOK2 protein isoform Alpha. This antibody mainly detects the isoforms 1, 3, and 4. It does not detect isoform 2.

Immunogen sequence: SLKVRAGQR PPGLPLPIPG ALGPPNTGTP IEQQPCSPGQ EAVLDQRMLG EEEEAVGERR ILGKEGATLE PKQQRILGEE SGAPSPSPQK HGSLVDEEVW GLPEEIEELR VPSLVPQERS IVGQEEAGTW SLWGKEDESL LDEEFELGWV QGPALTPVPE EEEEEEEGAP IGTPRDPGDG CPSPDIPPEP PPTHLRPCPA SQLPGLLSHG LLAGLSFAVG SSSGLLPLL (742-969 aa encoded by BC142663)

Target Information

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEK ) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonist to the stress -responsive p38 MAPK (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.

Acta neuropathologica communications

Giacomini C,Koo CY,Yankova N,Tavares IA,Wray S,Noble W,Hanger DP,Morris JDH

Mon May 07 00:00:00 EDT 2018

A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.

Acta neuropathologica communications

Giacomini C,Koo CY,Yankova N,Tavares IA,Wray S,Noble W,Hanger DP,Morris JDH

Mon May 07 00:00:00 EDT 2018

Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.

American journal of human genetics

Burns DT,Donkervoort S,Müller JS,Knierim E,Bharucha-Goebel D,Faqeih EA,Bell SK,AlFaifi AY,Monies D,Millan F,Retterer K,Dyack S,MacKay S,Morales-Gonzalez S,Giunta M,Munro B,Hudson G,Scavina M,Baker L,Massini TC,Lek M,Hu Y,Ezzo D,AlKuraya FS,Kang PB,Griffin H,Foley AR,Schuelke M,Horvath R,Bönnemann CG

Thu May 03 00:00:00 EDT 2018

Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.

American journal of human genetics

Thu May 03 00:00:00 EDT 2018

Targeting TAO Kinases Using a New Inhibitor Compound Delays Mitosis and Induces Mitotic Cell Death in Centrosome Amplified Breast Cancer Cells.

Molecular cancer therapeutics

Koo CY,Giacomini C,Reyes-Corral M,Olmos Y,Tavares IA,Marson CM,Linardopoulos S,Tutt AN,Morris JDH

Wed Nov 01 00:00:00 EDT 2017

Targeting TAO Kinases Using a New Inhibitor Compound Delays Mitosis and Induces Mitotic Cell Death in Centrosome Amplified Breast Cancer Cells.

Molecular cancer therapeutics

Koo CY,Giacomini C,Reyes-Corral M,Olmos Y,Tavares IA,Marson CM,Linardopoulos S,Tutt AN,Morris JDH

Wed Nov 01 00:00:00 EDT 2017

Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate tau protein and are activated in tangle-bearing neurons in Alzheimer disease.

The Journal of biological chemistry

Tavares IA,Touma D,Lynham S,Troakes C,Schober M,Causevic M,Garg R,Noble W,Killick R,Bodi I,Hanger DP,Morris JD

Fri May 24 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: hKFC-C; Kinase from chicken homolog C; prostate derived STE20-like kinase PSK; prostate-derived STE20-like kinase 1; Prostate-derived sterile 20-like kinase 1; PSK-1; serine-threonine kinase; serine/threonine protein kinase TAO2; Serine/threonine-protein kinase TAO2; tao; TAO kinase 2TAO kinase 2; Thousand and one amino acid protein 2; Thousand and one amino acid protein kinase 2

Gene Aliases: 1110033K02Rik; B230344N16; KIAA0881; MAP3K17; mKIAA0881; PSK; PSK1; PSK1-BETA; TAO1; TAO2; TAOK2; UNQ2971/PRO7431

UniProt ID: (Human) Q9UL54, (Mouse) Q6ZQ29, (Rat) Q9JLS3

Entrez Gene ID: (Human) 9344, (Mouse) 381921, (Rat) 64666

Molecular Function: non-receptor serine/threonine protein kinase