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Invitrogen
TAP Polyclonal Antibody
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FIGURE: 1 / 4
TAP Antibody (PA5-104491) in ICC/IF
Immunofluorescent analysis of TAP in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TAP polyclonal antibody (Product # PA5-104491) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-104491
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NXF1(Accession Q9UBU9), corresponding to amino acid residues M1-R38.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NXF1.
Target Information
The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the Nuclear Transport Factor 2 (NTF2)-like domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mex 67 homolog; modifier of vibrator 1; mRNA export factor TAP; Nuclear RNA export factor 1; nuclear RNA export factor 1 homolog; tip associated protein; tip associating protein; Tip-associated protein; Tip-associating protein; Vdp
Gene Aliases: MEX67; Mvb1; NXF1; TAP
UniProt ID: (Human) Q9UBU9, (Mouse) Q99JX7
Entrez Gene ID: (Human) 10482, (Mouse) 53319
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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