Proteintech

TAP1 Polyclonal Antibody

Product Image Gallery

TAP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using TAP1 antibody 11114-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TAP1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TAP1 Antibody in Immunoprecipitation (IP) — IP result of anti-TAP1 (IP:11114-1-AP, 4ug; Detection:11114-1-AP 1:500) with HepG2 cells lysate 2400ug.

Product Details

11114-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TAP1 Fusion Protein Ag1619 (509-808 aa encoded by BC014081) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Western blot analysis using this antibody detected a major band around 70-80kD in HeLa cells.

Immunogen sequence: LYQMQFTQA VEVLLSIYPR VQKAVGSSEK IFEYLDRTPR CPPSGLLTPL HLEGLVQFQD VSFAYPNRPD VLVLQGLTFT LRPGEVTALV GPNGSGKSTV AALLQNLYQP TGGQLLLDGK PLPQYEHRYL HRQVAAVGQE PQVFGRSLQE NIAYGLTQKP TMEEITAAAV KSGAHSFISG LPQGYDTEVD EAGSQLSGGQ RQAVALARAL IRKPCVLILD DATSALDANS QLQVEQLLYE SPERYSRSVL LITQHLSLVE QADHILFLEG GAIREGGTHQ QLMEKKGCYW AMVQAPADAP E (509-808 aa encoded by BC014081)

Target Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.

Photodiagnosis and photodynamic therapy

Ogihara K,Isomoto H,Kurumi H,Kanda T,Hashisako M,Tabata K,Ishii H,Ohnita K,Yamaguchi N,Akazawa Y,Matsushima K,Takeshima F,Kunizaki M,Hidaka S,Nanashima A,Fukuoka J,Nagayasu T,Nakao K

Fri Sep 01 00:00:00 EDT 2017

IRG1 increases MHC class I level in macrophages through STAT-TAP1 axis depending on NADPH oxidase mediated reactive oxygen species.

International immunopharmacology

Liu X,Wu XP,Zhu XL,Li T,Liu Y

Sat Jul 01 00:00:00 EDT 2017

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Journal of immunology (Baltimore, Md. : 1950)

Xia Z,Xu G,Yang X,Peng N,Zuo Q,Zhu S,Hao H,Liu S,Zhu Y

Mon May 01 00:00:00 EDT 2017

Characterization of the platelet granule proteome: evidence of the presence of MHC1 in alpha-granules.

Journal of proteomics

Zufferey A,Schvartz D,Nolli S,Reny JL,Sanchez JC,Fontana P

Mon Apr 14 00:00:00 EDT 2014

Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.

Asian Pacific journal of cancer prevention : APJCP

Zhao X,Sun Q,Tian H,Cong B,Jiang X,Peng C

Thu Jun 28 00:00:00 EDT 2012

The unfolded protein response (UPR)-activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes.

The Journal of biological chemistry

Bartoszewski R,Brewer JW,Rab A,Crossman DK,Bartoszewska S,Kapoor N,Fuller C,Collawn JF,Bebok Z

Fri Dec 02 00:00:00 EST 2011

The Journal of biological chemistry

Bartoszewski R,Brewer JW,Rab A,Crossman DK,Bartoszewska S,Kapoor N,Fuller C,Collawn JF,Bebok Z

Fri Dec 02 00:00:00 EST 2011

Methylation of the promoter of human leukocyte antigen class I in human esophageal squamous cell carcinoma and its histopathological characteristics.

The Journal of thoracic and cardiovascular surgery

Qifeng S,Bo C,Xingtao J,Chuanliang P,Xiaogang Z

Tue Mar 01 00:00:00 EST 2011

Down-regulation of the antigen processing machinery is linked to a loss of inflammatory response in colorectal cancer.

Human pathology

Kasajima A,Sers C,Sasano H,Jöhrens K,Stenzinger A,Noske A,Buckendahl AC,Darb-Esfahani S,Müller BM,Budczies J,Lehman A,Dietel M,Denkert C,Weichert W

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: ABC transporter, MHC 1; ABC transporter, MHC, 1; Antigen peptide transporter 1; AOI; APT1; ATP dependent transport protein family member; ATP-binding cassette sub-family B member 2; ATP-binding cassette transporter, major histocompatibility complex, 1; ATP-binding cassette, sub-family B (MDR/TAP), member 2; beta-filamin; DAAP-57C1.5; FH1; filamin homolog 1; filamin-3; filamin-B; FLJ26666; FLJ41500; FLN1L; Histocompatibility antigen modifier 1; LRS1; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF1; Peptide transporter TAP1; PSF-1; Really interesting new gene 4 protein; RING4; TABP; tap i; thyroid autoantigen; transporter 1 ABC (ATP binding cassette); transporter 1 ATP-binding cassette sub-family B; transporter 1, ABC (ATP binding cassette); transporter 1, ATP-binding cassette, sub-family B (MDR/TAP); transporter associated with antigen processing; transporter associated with antigen processing 1; transporter, ABC, MHC, 1; transporter, ATP-binding cassette, major histocompatibility complex, 1

Gene Aliases: ABC17; ABCB2; APT1; Cim; D6S114E; Ham-1; Ham1; MTP1; PSF-1; PSF1; RING4; TAP; Tap-1; TAP1; TAP1*0102N; TAP1N; Tap2; Y3

UniProt ID: (Human) Q03518, (Mouse) P21958

Entrez Gene ID: (Human) 6890, (Mouse) 21354, (Rat) 24811

Molecular Function: ATP-binding cassette (ABC) transporter