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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TARS2 Antibodies

          Invitrogen

          TARS2 Polyclonal Antibody

          View all (5) TARS2 antibodies

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          Cite TARS2 Polyclonal Antibody

          • Antibody Testing Data (8)
          TARS2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TARS2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          TARS2 Antibody (PA5-144027) in ICC/IF

          Immunocytochemistry analysis of TARS2 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TARS2 Polyclonal antibody (Product # PA5-144027) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TARS2 Antibody in Immunocytochemistry (ICC/IF)
          TARS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TARS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TARS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TARS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TARS2 Antibody in Western Blot (WB)
          TARS2 Antibody in Flow Cytometry (Flow)
          TARS2 Antibody in Flow Cytometry (Flow)
          TARS2 Polyclonal Antibody

          Product Details

          PA5-144027

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human TARS2 recombinant protein (Position: Q69-F718).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075241

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human HepG2 whole cell, human Hela whole cell, human THP-1 whole cell, human Raji whole cell, rat liver tissue, mouse liver tissue. IHC: human colonic adenocarcinoma tissue, human lung cancer tissue, rat brain tissue, rat brain tissue. ICC/IF: T-47D cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Threonine--tRNA ligase, mitochondrial; Threonyl-tRNA synthetase; threonyl-tRNA synthetase, mitochondrial; Threonyl-tRNA synthetase-like 1; ThrRS

          View more View less

          Gene Aliases: 2610024N01Rik; AI429208; COXPD21; RGD1308283; TARS2; TARSL1; thrRS

          View more View less

          UniProt ID: (Human) Q9BW92, (Mouse) Q3UQ84, (Rat) Q68FW7

          View more View less

          Entrez Gene ID: (Human) 80222, (Mouse) 71807, (Rat) 310672

          View more View less

          Function(s)
          molecular_function RNA binding threonine-tRNA ligase activity ATP binding nucleotide binding aminoacyl-tRNA ligase activity ligase activity ligase activity, forming aminoacyl-tRNA and related compounds aminoacyl-tRNA synthetase
          Process(es)
          mitochondrial threonyl-tRNA aminoacylation translation tRNA aminoacylation for protein translation threonyl-tRNA aminoacylation tRNA aminoacylation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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