Invitrogen

TBP Polyclonal Antibody

Product Image Gallery

TBP Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of TBP in 293 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116009) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

TBP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TBP in human kidney cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116009) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

TBP Antibody in Western Blot (WB) — Western blot analysis of TBP in MCF7 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-116009).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human TBP, corresponding to a region within N-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

UniProt ID: (Human) P20226, (Mouse) P29037

Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

Molecular Function: general transcription factor