|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding D(136) P K T L A R L N D M K E K P I C(152) from human TC10.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1061 detects human and mouse TC10 protein in transfected cell samples and endogenous levels in tissue extracts. PA1-1061 is specific for isotype TC10a and demonstrates slight cross-reactivity with TC10b.
PA1-1061 has successfully been used in immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~24 kDa protein representing TC10 in transfected COS cells and a non-specific, unidentified band at ~50 kDa.
The PA1-1061 immunogen is a synthetic peptide corresponding D(136) P K T L A R L N D M K E K P I C(152) from human TC10. This peptide (Cat. # PEP-219) is available for use in neutralization and control experiments.
TC10 has been classified as a member of the Rho family of GTPases and possesses characteristics most closely related to Cdc42. This family of proteins regulates a wide variety of cellular activity including cytoskeletal rearrangements, motility, proliferation and apoptosis. TC10 is predominately localized to the cell membrane and TC10 mRNA was most highly expressed in heart and skeletal muscle.
Recent studies with TC10 mutations have demonstrated that TC10 is responsible for the activation of c-jun amino terminal kinase, the activation of serum response factor-dependent transcription, and the formation of long filopodia. In other studies, TC10 interacts with a similar subset of effectors as Cdc42, but does not react with WASP, MLK3, or ACK-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.