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Invitrogen
TCEB2 Polyclonal Antibody
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FIGURE: 1 / 7
TCEB2 Antibody (PA5-144068) in ICC/IF
Immunocytochemistry analysis of TCEB2 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TCEB2 Polyclonal antibody (Product # PA5-144068) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More
Product Details
PA5-144068
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TCEB2/Elongin-B/ELOB recombinant protein (Position: M1-Q118).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human Hela whole cell. IHC: mouse pancreas tissue, rat pancreas tissue, human papillary carcinoma of the left breast tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa, elongin B; EloB; Elongin 18 kDa subunit; elongin B; elongin, 18-kD subunit; Elongin-B; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII p18; TCEB 2; transcription elongation factor B (SIII) polypeptide 2 (18kD, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18 kDa, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Transcription elongation factor B polypeptide 2
Gene Aliases: 0610040H15Rik; ELOB; SIII; TCEB2
UniProt ID: (Human) Q15370, (Mouse) P62869, (Rat) P62870
Entrez Gene ID: (Human) 6923, (Mouse) 67673, (Rat) 81807
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein complex assembly
protein ubiquitination
regulation of transcription from RNA polymerase II promoter in response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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