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TCF12 Antibody (A300-755A) in WB
Detection of human TCF12 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TCF12 antibody BL2271 (Cat. No. A300-755A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody BL2270 (Cat. No. A300-754A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) an... View More
Product Details
A300-755A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 and 100 of Human Transcription Factor 12.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb20; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; helix-loop-helix transcription factor 4; TCF-12; Transcription factor 12; Transcription factor HTF-4
Gene Aliases: BHLHB20; CRS3; HEB; HsT17266; HTF4; TCF-12; TCF12
UniProt ID: (Human) Q99081
Entrez Gene ID: (Human) 6938
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
enhancer binding
cAMP response element binding
protein homodimerization activity
bHLH transcription factor binding
transcription regulatory region DNA binding
SMAD binding
protein heterodimerization activity
E-box binding
HMG box domain binding
basic helix-loop-helix transcription factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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