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Invitrogen
TCF2 Polyclonal Antibody
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FIGURE: 1 / 5
TCF2 Antibody (720259) in ICC/IF
For immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous HNF1b using HNF1b Rabbit Polyclonal Antibody (Product# 720259, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HNF1b protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) repr... View More
Product Details
720259
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human HNF1b (aa 511:528, 191:208)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Horse and Cat.
Target Information
Hepatocyte Nuclear Factor 1 (HNF1, Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor) is a member of the homeodomain-containing superfamily of transcription factors. It is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. HNF1has been shown to function in nephron development and regulates development of the embryonic pancreas. Mutations in the gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of the gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Hepatocyte nuclear factor 1-beta; hepatocyte nuclear factor-1 beta; HNF-1-beta; HNF-1B; HNF1 beta A; Homeoprotein LFB3; TCF-2; TCF2; transcription factor; Transcription factor 2; Transcription factor 2 hepatic; Transcription factor 2 hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2, hepatic; Transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; unnamed protein product; Variant hepatic nuclear factor 1; vHNF1
Gene Aliases: ADTKD3; FJHN; HNF-1-beta; HNF-1B; HNF-1Beta; HNF1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; RCAD; T2D; TCF-2; TCF2; VHNF1
UniProt ID: (Human) P35680, (Rat) P23899, (Mouse) P27889
Entrez Gene ID: (Human) 6928, (Rat) 25640, (Mouse) 21410
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription cofactor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
protein homodimerization activity
promoter-specific chromatin binding
core promoter proximal region DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein complex binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
kidney development
inner cell mass cell differentiation
liver development
regulation of transcription from RNA polymerase II promoter
apoptotic process
Notch signaling pathway
endoderm development
response to glucose
anterior/posterior pattern specification
gene expression
positive regulation of gene expression
insulin secretion
regulation of Wnt signaling pathway
hindbrain development
pancreas development
endocrine pancreas development
regulation of pronephros size
pronephric nephron tubule development
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
animal organ development
embryonic digestive tract morphogenesis
system development
branching morphogenesis of an epithelial tube
pronephros development
genitalia development
epithelial cell proliferation
positive regulation of transcription initiation from RNA polymerase II promoter
epithelium development
ureteric bud elongation
kidney morphogenesis
hepatoblast differentiation
negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis
regulation of branch elongation involved in ureteric bud branching
mesonephric tubule development
nephric duct development
mesonephric duct development
nephric duct formation
mesonephric duct formation
mesenchymal cell apoptotic process involved in metanephros development
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
transcription from RNA polymerase II promoter
response to carbohydrate
response to organic cyclic compound
circadian regulation of gene expression
response to drug
regulation of endodermal cell fate specification
embryonic morphogenesis
protein-DNA complex assembly
hepatocyte differentiation
endoderm formation
transcription, DNA-templated
regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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