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Invitrogen

TCF3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

1 Reference
View all (18) TCF3 antibodies
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Product Details

Applications
Tested Dilution
Publications
Western Blot (WB)
1 µg/mL
View 1 publication 1 publication
Immunohistochemistry (IHC)
5 µg/mL
-
Product Specifications
Species Reactivity
Human, Mouse, Rat
Published species
Human
Host/Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide near the amino terminus of human TCF3.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID
AB_11152186

Product Specific Information

A suggested positive control is human brain tissue lysate.

PA5-20900 can be used with blocking peptide PEP-1014.

Target Information

TCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bHLHb21; Class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; MGC129647; MGC129648; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; Pancreas specific transcription factor 1c; TCF-3; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor A1; Transcription factor E2-alpha; transcription factor E2a; Transcription factor ITF-1; Transcription regulator Pan; VDR interacting repressor; vitamin D receptor-interacting repressor

Gene Aliases: A1; AA408400; AGM8; ALF2; AW209082; BHLHB21; E12; E12/E47; E2A; E47; ITF1; ME2; Pan; Pan1; Pan2; Ptf1c; TCF-3; TCF3; Tcfe2a; VDIR

UniProt ID: (Human) P15923, (Mouse) P15806, (Rat) P21677

Entrez Gene ID: (Human) 6929, (Mouse) 21423, (Rat) 171046

Molecular Function: basic helix-loop-helix transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

Function(s)
negative regulation of transcription from RNA polymerase II promoter B cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated B cell differentiation immunoglobulin V(D)J recombination positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of muscle cell differentiation natural killer cell differentiation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter gastrulation nervous system development positive regulation of gene expression lymphocyte differentiation cell differentiation positive regulation of B cell proliferation response to lipopolysaccharide T cell differentiation in thymus response to drug histone H3 acetylation histone H4 acetylation positive regulation of cell cycle cell development Peyer's patch development protein stabilization regulation of G1/S transition of mitotic cell cycle
Process(es)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding mitogen-activated protein kinase kinase kinase binding enhancer binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity repressing transcription factor binding vitamin D response element binding E-box binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding chromatin binding PDZ domain binding sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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