Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TCF4/TCF12 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
TCF4/TCF12 Antibody (PA5-36151) in WB
Western blot analysis of TCF4/TCF12 in Lane 1: SGC7901 whole cell lysate (20 µg), Lane 2: HepG2 whole cell lysate (40 µg), Lane 3: Jurkat whole cell lysate (40 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: PMVEC whole cell lysate (40 µg), Lane 6: AML-12 whole cell lysate (40 µg). Samples were incubated with TCF4/TCF12 polyclonal antibody (Product # PA5-36151) at a dilution of 1:500.
Product Details
PA5-36151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 580-630 of human TCF-4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 60 and 72 kDa.
Purity is >95% by SDS-PAGE.
Target Information
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group(HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/ beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: bHLHb19; bHLHb20; Class A helix-loop-helix transcription factor ME1; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; helix-loop-helix transcription factor 4; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF-2; MITF-2; R8f DNA-binding protein; RITF-2; Salivary-specific cAMP response element-binding protein alpha; SCBP alpha; SCBP-alpha; SEF-2; SL3-3 enhancer factor 2; TCF-12; TCF-4; Transcription factor 12; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R; Transcription factor HTF-4
Gene Aliases: 5730422P05Rik; A130037E08Rik; ALF1; ASP-I2; BHLHB19; BHLHB20; CRS3; E2-2; E2.2; FECD3; HEB; HEBAlt; HsT17266; HTF-4; HTF4; ITF-2; ITF-2b; ITF2; ME1; ME2; Meb; MITF-2A; MITF-2B; PTHS; REB; SCBPA; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-12; TCF-4; TCF12; TCF4; TFE
UniProt ID: (Human) Q99081, (Human) P15884, (Mouse) Q61286, (Rat) P51514, (Mouse) Q60722, (Rat) Q62655
Entrez Gene ID: (Human) 6938, (Human) 6925, (Mouse) 21406, (Rat) 25720, (Mouse) 21413, (Rat) 84382
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
enhancer binding
cAMP response element binding
protein homodimerization activity
bHLH transcription factor binding
transcription regulatory region DNA binding
SMAD binding
protein heterodimerization activity
E-box binding
HMG box domain binding
transcription factor activity, sequence-specific DNA binding, RNA polymerase recruiting
transcription factor activity, TFIIB-class binding
TFIIB-class transcription factor binding
DNA binding
protein C-terminus binding
identical protein binding
protein dimerization activity
chromatin binding
double-stranded DNA binding
transcription corepressor activity
basic helix-loop-helix transcription factor
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
immune response
muscle organ development
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
DNA-templated transcription, initiation
transcription initiation from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
protein-DNA complex assembly
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
nervous system development
positive regulation of gene expression
cell differentiation
negative regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support