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Invitrogen
TCF7 Polyclonal Antibody
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FIGURE: 1 / 1
TCF7 Antibody (PA5-91923) in WB
Western blot analysis of TCF7 in extracts of various cell lines. Samples were incubated with TCF7 Polyclonal antibody (Product # PA5-91923) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-91923
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCF1/TCF7 (NP_0031932)
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse spleen; Cellular Location: Nucleus
Immunogen sequence: ELKSSLVNES EGAAGGAGIP GVPGAGAGAR GEAEALGREH AAQRLFPDKL PEPLEDGLKA PECTSGMYKE TVYSAFNLLM HYPPPSGAGQ HPQPQPPLHK A
Target Information
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T cell factor-1; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7; transcription factor 7, T-cell specific
Gene Aliases: AI465550; TCF-1; TCF1; TCF7
UniProt ID: (Human) P36402, (Mouse) Q00417
Entrez Gene ID: (Human) 6932, (Rat) 363595, (Mouse) 21414
core promoter binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
beta-catenin binding
sequence-specific DNA binding
transcription regulatory region DNA binding
molecular_function
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
neural tube development
embryonic genitalia morphogenesis
T cell receptor V(D)J recombination
regulation of cell proliferation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
alpha-beta T cell differentiation
embryonic digestive tract morphogenesis
embryonic hindgut morphogenesis
canonical Wnt signaling pathway
cellular response to interleukin-4
beta-catenin-TCF complex assembly
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
brain development
biological_process
Wnt signaling pathway
generation of neurons
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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