Invitrogen

TCP1 Monoclonal Antibody (91a), FITC

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TCP1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with TCP1-alpha monoclonal antibody (Product # MA5-45719) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha antibody. (C) Composite. Heat Shocked at 42°C for 1h.

TCP1 Antibody in Western Blot (WB) — Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein. Samples were incubated with TCP1 alpha monoclonal antibody (Product # MA5-45719) at 1:1,000.

Product Details

MA5-45719

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Bovine, Dog, C. elegans, Fruit fly, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat, Yeast
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	91a
Immunogen	Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Conjugate	FITC APC PerCP
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	4° C, store in dark

Target Information

The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 65 kDa antigen; CCT-alpha; chaperonin-containing T-complex alpha subunit TCP1; chaperonin-containing T-complex polypeptide alpha subunit; cytosolic chaperonin containing t-complex polypeptide 1; t-complex 1; t-complex polypeptide 1; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha A; T-complex protein 1 subunit alpha B; T-complex protein 1, alpha subunit; tailless complex polypeptide 1; Tailless complex polypeptide 1A; Tailless complex polypeptide 1B; TCP-1-A; TCP-1-alpha; TCP-1-B

Gene Aliases: AI528772; c-cpn; CCT; CCT-alpha; CCT1; CCTA; CCTalpha; D6S230E; p63; Tcp-1; TCP-1-alpha; TCP1; Tp63; TRiC; YD8142.13; YD8142B.04; YDR212W

UniProt ID: (Baker's yeast) P12612, (Bovine) Q32L40, (Human) P17987, (Rat) P28480, (Mouse) P11983, (Chinese hamster) P18279

Entrez Gene ID: (Guinea pig) 100713821, (Baker's yeast) 851798, (Rabbit) 100302435, (Dog) 484064, (Pig) 100153485, (Bovine) 512043, (Human) 6950, (Rat) 24818, (Mouse) 21454, (Chinese hamster) 100689476