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Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Flow Cytometry (Flow) |
Assay-dependent | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Dog, C. elegans, Fruit fly, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat, Yeast |
Host/Isotype |
Rat / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
91a |
Immunogen |
Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region). |
Conjugate |
FITC
|
Excitation/Emission Max |
498/517 nm View spectra |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
9.44mM PBS/9.1mM sodium bicarbonate, pH 7.4, with 581mM DMSO, 123mM ethanolamine |
Contains |
no preservative |
Storage conditions |
4° C, store in dark |
The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Protein Aliases: 65 kDa antigen; CCT-alpha; chaperonin-containing T-complex alpha subunit TCP1; chaperonin-containing T-complex polypeptide alpha subunit; cytosolic chaperonin containing t-complex polypeptide 1; t-complex 1; t-complex polypeptide 1; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha A; T-complex protein 1 subunit alpha B; T-complex protein 1, alpha subunit; tailless complex polypeptide 1; Tailless complex polypeptide 1A; Tailless complex polypeptide 1B; TCP-1-A; TCP-1-alpha; TCP-1-B
Gene Aliases: AI528772; c-cpn; CCT; CCT-alpha; CCT1; CCTA; CCTalpha; D6S230E; p63; Tcp-1; TCP-1-alpha; TCP1; Tp63; TRiC; YD8142.13; YD8142B.04; YDR212W
UniProt ID: (Baker's yeast) P12612, (Bovine) Q32L40, (Human) P17987, (Rat) P28480, (Mouse) P11983, (Chinese hamster) P18279
Entrez Gene ID: (Guinea pig) 100713821, (Baker's yeast) 851798, (Rabbit) 100302435, (Dog) 484064, (Pig) 100153485, (Bovine) 512043, (Human) 6950, (Rat) 24818, (Mouse) 21454, (Chinese hamster) 100689476
Molecular Function:
chaperonin
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